Transferring:Article Title: Ex vivo Analysis of DNA Repair Capacity of Human Peripheral Blood Mononuclear Cells by a Modified Host Cell Reactivation Assay
Article Snippet: .. Centrifuge tubes 15, 50 ml (SARSTEDT, catalog numbers: 62.554.001, 62.547.254) 1.5 ml reaction tube (SARSTEDT, catalog number: 72.706) Petri dishes (SARSTEDT, catalog number: 83.3902) Pipette tips 10 μl, 20 μl, 200 μl, 1,000 μl (SARSTEDT, catalog numbers: 70.1130, 70.116, 70.760.002, 70.762) Serological pipettes 5 ml and 10 ml (SARSTEDT, catalog numbers: 86.1253.001 and 86.1254.001) Leucosep TM , with porous barrier, 50 ml, pre filled with Leucosep TM separation medium (Greiner Bio-One GmbH, catalog number: 227288) Sterile filter 0.2 μm (SARSTEDT, catalog number: 83.1826.001) Cell culture plate, 6-well, Cell+ (SARSTEDT, catalog number: 83.3920.300) 0.4 cm electroporation cuvettes (Molecular BioProducts, Inc., purchased via Thermo Fisher Scientific GmbH, catalog number: 5540-11) FACS tubes 5 ml and 0.5 ml (SARSTEDT, catalog numbers: 55.1579, 55.673) Plasmid-DNA solution pEGFP-N1 (Clontech, catalog number: 6085-1) and pDsRedExpress-N1 (Clontech, catalog number: 632429), each 900 ng/μl in Qiagen EB buffer (storage temperature for longer periods: -80 °C, otherwise keep at 4 °C) Human venous blood, anticoagulated KCl (Carl Roth, catalog number: 6781.3) KH 2 PO 4 (Carl Roth, catalog number: 3904.2) Na 2 HPO 4 ·12H 2 O (Carl Roth, catalog number: N350.1) NaCl (Carl Roth, catalog number: 3957.3) S-Monovette ® K3 EDTA 4.9 ml and 7.5 ml (SARSTEDT, catalog numbers: 04.1931.001 and 01.1605.001) RPMI 1640 without Phenol Red (Gibco ® by Life Technologies, purchased via Fisher Scientific GmbH, catalog number: 11835-063, storage temp. .. 4 °C) Fetal Bovine Serum (Gibco ® by Life Technologies, chased via Fisher Scientific GmbH, catalog number: 10270-106) EB Buffer, part of the QIAGEN Plasmid Plus Giga Kit (Qiagen GmbH, Hilden, catalog number: 12991) BD FACS Sheath Solution with Surfactant (BD Biosciences, catalog number: 336911) 1x PBS (see Recipes) 0.9% NaCl solution (see Recipes) PBMC medium (see Recipes)
Sterility:Article Title: Ex vivo Analysis of DNA Repair Capacity of Human Peripheral Blood Mononuclear Cells by a Modified Host Cell Reactivation Assay
Article Snippet: .. Centrifuge tubes 15, 50 ml (SARSTEDT, catalog numbers: 62.554.001, 62.547.254) 1.5 ml reaction tube (SARSTEDT, catalog number: 72.706) Petri dishes (SARSTEDT, catalog number: 83.3902) Pipette tips 10 μl, 20 μl, 200 μl, 1,000 μl (SARSTEDT, catalog numbers: 70.1130, 70.116, 70.760.002, 70.762) Serological pipettes 5 ml and 10 ml (SARSTEDT, catalog numbers: 86.1253.001 and 86.1254.001) Leucosep TM , with porous barrier, 50 ml, pre filled with Leucosep TM separation medium (Greiner Bio-One GmbH, catalog number: 227288) Sterile filter 0.2 μm (SARSTEDT, catalog number: 83.1826.001) Cell culture plate, 6-well, Cell+ (SARSTEDT, catalog number: 83.3920.300) 0.4 cm electroporation cuvettes (Molecular BioProducts, Inc., purchased via Thermo Fisher Scientific GmbH, catalog number: 5540-11) FACS tubes 5 ml and 0.5 ml (SARSTEDT, catalog numbers: 55.1579, 55.673) Plasmid-DNA solution pEGFP-N1 (Clontech, catalog number: 6085-1) and pDsRedExpress-N1 (Clontech, catalog number: 632429), each 900 ng/μl in Qiagen EB buffer (storage temperature for longer periods: -80 °C, otherwise keep at 4 °C) Human venous blood, anticoagulated KCl (Carl Roth, catalog number: 6781.3) KH 2 PO 4 (Carl Roth, catalog number: 3904.2) Na 2 HPO 4 ·12H 2 O (Carl Roth, catalog number: N350.1) NaCl (Carl Roth, catalog number: 3957.3) S-Monovette ® K3 EDTA 4.9 ml and 7.5 ml (SARSTEDT, catalog numbers: 04.1931.001 and 01.1605.001) RPMI 1640 without Phenol Red (Gibco ® by Life Technologies, purchased via Fisher Scientific GmbH, catalog number: 11835-063, storage temp. .. 4 °C) Fetal Bovine Serum (Gibco ® by Life Technologies, chased via Fisher Scientific GmbH, catalog number: 10270-106) EB Buffer, part of the QIAGEN Plasmid Plus Giga Kit (Qiagen GmbH, Hilden, catalog number: 12991) BD FACS Sheath Solution with Surfactant (BD Biosciences, catalog number: 336911) 1x PBS (see Recipes) 0.9% NaCl solution (see Recipes) PBMC medium (see Recipes)
Cell Culture:Article Title: Ex vivo Analysis of DNA Repair Capacity of Human Peripheral Blood Mononuclear Cells by a Modified Host Cell Reactivation Assay
Article Snippet: .. Centrifuge tubes 15, 50 ml (SARSTEDT, catalog numbers: 62.554.001, 62.547.254) 1.5 ml reaction tube (SARSTEDT, catalog number: 72.706) Petri dishes (SARSTEDT, catalog number: 83.3902) Pipette tips 10 μl, 20 μl, 200 μl, 1,000 μl (SARSTEDT, catalog numbers: 70.1130, 70.116, 70.760.002, 70.762) Serological pipettes 5 ml and 10 ml (SARSTEDT, catalog numbers: 86.1253.001 and 86.1254.001) Leucosep TM , with porous barrier, 50 ml, pre filled with Leucosep TM separation medium (Greiner Bio-One GmbH, catalog number: 227288) Sterile filter 0.2 μm (SARSTEDT, catalog number: 83.1826.001) Cell culture plate, 6-well, Cell+ (SARSTEDT, catalog number: 83.3920.300) 0.4 cm electroporation cuvettes (Molecular BioProducts, Inc., purchased via Thermo Fisher Scientific GmbH, catalog number: 5540-11) FACS tubes 5 ml and 0.5 ml (SARSTEDT, catalog numbers: 55.1579, 55.673) Plasmid-DNA solution pEGFP-N1 (Clontech, catalog number: 6085-1) and pDsRedExpress-N1 (Clontech, catalog number: 632429), each 900 ng/μl in Qiagen EB buffer (storage temperature for longer periods: -80 °C, otherwise keep at 4 °C) Human venous blood, anticoagulated KCl (Carl Roth, catalog number: 6781.3) KH 2 PO 4 (Carl Roth, catalog number: 3904.2) Na 2 HPO 4 ·12H 2 O (Carl Roth, catalog number: N350.1) NaCl (Carl Roth, catalog number: 3957.3) S-Monovette ® K3 EDTA 4.9 ml and 7.5 ml (SARSTEDT, catalog numbers: 04.1931.001 and 01.1605.001) RPMI 1640 without Phenol Red (Gibco ® by Life Technologies, purchased via Fisher Scientific GmbH, catalog number: 11835-063, storage temp. .. 4 °C) Fetal Bovine Serum (Gibco ® by Life Technologies, chased via Fisher Scientific GmbH, catalog number: 10270-106) EB Buffer, part of the QIAGEN Plasmid Plus Giga Kit (Qiagen GmbH, Hilden, catalog number: 12991) BD FACS Sheath Solution with Surfactant (BD Biosciences, catalog number: 336911) 1x PBS (see Recipes) 0.9% NaCl solution (see Recipes) PBMC medium (see Recipes)
Electroporation:Article Title: Ex vivo Analysis of DNA Repair Capacity of Human Peripheral Blood Mononuclear Cells by a Modified Host Cell Reactivation Assay
Article Snippet: .. Centrifuge tubes 15, 50 ml (SARSTEDT, catalog numbers: 62.554.001, 62.547.254) 1.5 ml reaction tube (SARSTEDT, catalog number: 72.706) Petri dishes (SARSTEDT, catalog number: 83.3902) Pipette tips 10 μl, 20 μl, 200 μl, 1,000 μl (SARSTEDT, catalog numbers: 70.1130, 70.116, 70.760.002, 70.762) Serological pipettes 5 ml and 10 ml (SARSTEDT, catalog numbers: 86.1253.001 and 86.1254.001) Leucosep TM , with porous barrier, 50 ml, pre filled with Leucosep TM separation medium (Greiner Bio-One GmbH, catalog number: 227288) Sterile filter 0.2 μm (SARSTEDT, catalog number: 83.1826.001) Cell culture plate, 6-well, Cell+ (SARSTEDT, catalog number: 83.3920.300) 0.4 cm electroporation cuvettes (Molecular BioProducts, Inc., purchased via Thermo Fisher Scientific GmbH, catalog number: 5540-11) FACS tubes 5 ml and 0.5 ml (SARSTEDT, catalog numbers: 55.1579, 55.673) Plasmid-DNA solution pEGFP-N1 (Clontech, catalog number: 6085-1) and pDsRedExpress-N1 (Clontech, catalog number: 632429), each 900 ng/μl in Qiagen EB buffer (storage temperature for longer periods: -80 °C, otherwise keep at 4 °C) Human venous blood, anticoagulated KCl (Carl Roth, catalog number: 6781.3) KH 2 PO 4 (Carl Roth, catalog number: 3904.2) Na 2 HPO 4 ·12H 2 O (Carl Roth, catalog number: N350.1) NaCl (Carl Roth, catalog number: 3957.3) S-Monovette ® K3 EDTA 4.9 ml and 7.5 ml (SARSTEDT, catalog numbers: 04.1931.001 and 01.1605.001) RPMI 1640 without Phenol Red (Gibco ® by Life Technologies, purchased via Fisher Scientific GmbH, catalog number: 11835-063, storage temp. .. 4 °C) Fetal Bovine Serum (Gibco ® by Life Technologies, chased via Fisher Scientific GmbH, catalog number: 10270-106) EB Buffer, part of the QIAGEN Plasmid Plus Giga Kit (Qiagen GmbH, Hilden, catalog number: 12991) BD FACS Sheath Solution with Surfactant (BD Biosciences, catalog number: 336911) 1x PBS (see Recipes) 0.9% NaCl solution (see Recipes) PBMC medium (see Recipes)
FACS:Article Title: Ex vivo Analysis of DNA Repair Capacity of Human Peripheral Blood Mononuclear Cells by a Modified Host Cell Reactivation Assay
Article Snippet: .. Centrifuge tubes 15, 50 ml (SARSTEDT, catalog numbers: 62.554.001, 62.547.254) 1.5 ml reaction tube (SARSTEDT, catalog number: 72.706) Petri dishes (SARSTEDT, catalog number: 83.3902) Pipette tips 10 μl, 20 μl, 200 μl, 1,000 μl (SARSTEDT, catalog numbers: 70.1130, 70.116, 70.760.002, 70.762) Serological pipettes 5 ml and 10 ml (SARSTEDT, catalog numbers: 86.1253.001 and 86.1254.001) Leucosep TM , with porous barrier, 50 ml, pre filled with Leucosep TM separation medium (Greiner Bio-One GmbH, catalog number: 227288) Sterile filter 0.2 μm (SARSTEDT, catalog number: 83.1826.001) Cell culture plate, 6-well, Cell+ (SARSTEDT, catalog number: 83.3920.300) 0.4 cm electroporation cuvettes (Molecular BioProducts, Inc., purchased via Thermo Fisher Scientific GmbH, catalog number: 5540-11) FACS tubes 5 ml and 0.5 ml (SARSTEDT, catalog numbers: 55.1579, 55.673) Plasmid-DNA solution pEGFP-N1 (Clontech, catalog number: 6085-1) and pDsRedExpress-N1 (Clontech, catalog number: 632429), each 900 ng/μl in Qiagen EB buffer (storage temperature for longer periods: -80 °C, otherwise keep at 4 °C) Human venous blood, anticoagulated KCl (Carl Roth, catalog number: 6781.3) KH 2 PO 4 (Carl Roth, catalog number: 3904.2) Na 2 HPO 4 ·12H 2 O (Carl Roth, catalog number: N350.1) NaCl (Carl Roth, catalog number: 3957.3) S-Monovette ® K3 EDTA 4.9 ml and 7.5 ml (SARSTEDT, catalog numbers: 04.1931.001 and 01.1605.001) RPMI 1640 without Phenol Red (Gibco ® by Life Technologies, purchased via Fisher Scientific GmbH, catalog number: 11835-063, storage temp. .. 4 °C) Fetal Bovine Serum (Gibco ® by Life Technologies, chased via Fisher Scientific GmbH, catalog number: 10270-106) EB Buffer, part of the QIAGEN Plasmid Plus Giga Kit (Qiagen GmbH, Hilden, catalog number: 12991) BD FACS Sheath Solution with Surfactant (BD Biosciences, catalog number: 336911) 1x PBS (see Recipes) 0.9% NaCl solution (see Recipes) PBMC medium (see Recipes)
Plasmid Preparation:Article Title: Ex vivo Analysis of DNA Repair Capacity of Human Peripheral Blood Mononuclear Cells by a Modified Host Cell Reactivation Assay
Article Snippet: .. Centrifuge tubes 15, 50 ml (SARSTEDT, catalog numbers: 62.554.001, 62.547.254) 1.5 ml reaction tube (SARSTEDT, catalog number: 72.706) Petri dishes (SARSTEDT, catalog number: 83.3902) Pipette tips 10 μl, 20 μl, 200 μl, 1,000 μl (SARSTEDT, catalog numbers: 70.1130, 70.116, 70.760.002, 70.762) Serological pipettes 5 ml and 10 ml (SARSTEDT, catalog numbers: 86.1253.001 and 86.1254.001) Leucosep TM , with porous barrier, 50 ml, pre filled with Leucosep TM separation medium (Greiner Bio-One GmbH, catalog number: 227288) Sterile filter 0.2 μm (SARSTEDT, catalog number: 83.1826.001) Cell culture plate, 6-well, Cell+ (SARSTEDT, catalog number: 83.3920.300) 0.4 cm electroporation cuvettes (Molecular BioProducts, Inc., purchased via Thermo Fisher Scientific GmbH, catalog number: 5540-11) FACS tubes 5 ml and 0.5 ml (SARSTEDT, catalog numbers: 55.1579, 55.673) Plasmid-DNA solution pEGFP-N1 (Clontech, catalog number: 6085-1) and pDsRedExpress-N1 (Clontech, catalog number: 632429), each 900 ng/μl in Qiagen EB buffer (storage temperature for longer periods: -80 °C, otherwise keep at 4 °C) Human venous blood, anticoagulated KCl (Carl Roth, catalog number: 6781.3) KH 2 PO 4 (Carl Roth, catalog number: 3904.2) Na 2 HPO 4 ·12H 2 O (Carl Roth, catalog number: N350.1) NaCl (Carl Roth, catalog number: 3957.3) S-Monovette ® K3 EDTA 4.9 ml and 7.5 ml (SARSTEDT, catalog numbers: 04.1931.001 and 01.1605.001) RPMI 1640 without Phenol Red (Gibco ® by Life Technologies, purchased via Fisher Scientific GmbH, catalog number: 11835-063, storage temp. .. 4 °C) Fetal Bovine Serum (Gibco ® by Life Technologies, chased via Fisher Scientific GmbH, catalog number: 10270-106) EB Buffer, part of the QIAGEN Plasmid Plus Giga Kit (Qiagen GmbH, Hilden, catalog number: 12991) BD FACS Sheath Solution with Surfactant (BD Biosciences, catalog number: 336911) 1x PBS (see Recipes) 0.9% NaCl solution (see Recipes) PBMC medium (see Recipes)
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